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subworkflows_sequence_to_trimmed_fastq_and_hisat_alignments.cwl

APipe Tester edited this page Nov 29, 2021 · 1 revision

Documentation for sequence_to_trimmed_fastq_and_hisat_alignments.cwl

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Overview

sequence (bam or fastqs) to trimmed fastqs and HISAT alignments

Inputs

Name Label Description Type Secondary Files
unaligned ../types/sequence_data.yml#sequence_data
adapters File
adapter_trim_end string
adapter_min_overlap int
max_uncalled int
min_readlength int
read_group_id string
read_group_fields {'type': 'array', 'items': 'string'}
reference_index File ['.1.ht2', '.2.ht2', '.3.ht2', '.4.ht2', '.5.ht2', '.6.ht2', '.7.ht2', '.8.ht2']
strand ['null', {'type': 'enum', 'symbols': ['first', 'second', 'unstranded']}]
unzip_fastqs boolean?

Outputs

Name Label Description Type Secondary Files
fastqs File[]
aligned_bam File

Steps

Name CWL Run
sequence_to_fastq tools/sequence_to_fastq.cwl
trim_fastq tools/trim_fastq.cwl
hisat2_align tools/hisat2_align.cwl
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