Uploading Data Files

The interface is equipped with a file-upload window at the very top which can be expanded upon clicking. The program accepts one normaliser file and any number of target files.

The qpcr package, which is the functional core of this program, is designed to work with csv files with a two-column data formatting and will only function properly if the formatting is fulfilled. This was chosen to work directly with qPCR data generated by Qiagen RotorGene®, which exports Excel sheets of this kind of formatting. The package is also designed to work directly with the raw CT values so there is no need to pre-compute the average of each group of replicates!

Sample	CT
geneX_1	10.6
geneX_2	10.9
geneX_3	10.2
geneY_1	5.8
...	…