Combined Normalisers
Often one single normaliser assay (such as Actin or 28S rRNA) are not adequate for your normalisation. In this case this program offers you to combine (=average) any number of input files and return a combined dataset.
To do so, you must load one normaliser and any number of target files which you wish to combine into the normaliser. You must also specify the same parameters that you will later use for your actual analysis – especially the groups and group names have to be identical to the ones you will use for analysis.
Once you have loaded the datasets you like and specified your parameters you may press the "Combine Normalisers" button:
During the computation the datasets will be pre-processed and analysed by Single DeltaCt. Once this is complete their group entries will be averaged. Then each group will be given n (n = replicate) entries of that combined average to return the original dimensionality of the dataset. The combined normaliser will be shown below alongside with a download link for the csv file.
Once you have prepared your combined normaliser you may load it as normaliser. Thereafter it is essential to check the "Uses combined normalisers" option. This is necessary as the combined normaliser has already been processed and does not need to undergo the same procedure again.
