Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

FIX: Better coverage in _all variant calling subworkflows #1216

Merged
merged 4 commits into from
Sep 8, 2023
Merged
Show file tree
Hide file tree
Changes from all commits
Commits
File filter

Filter by extension

Filter by extension

Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
1 change: 1 addition & 0 deletions CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -61,6 +61,7 @@ Rapaselet is a delta formed by the Rapaätno river between the Bielloriehppe mas
- [#1205](https://github.com/nf-core/sarek/pull/1205) - Less tests triggered
- [#1214](https://github.com/nf-core/sarek/pull/1214) - Don't pass in intervals file to ControlFREEC for WGS analysis
- [#1215](https://github.com/nf-core/sarek/pull/1215) - Fix `meta.id` for mutect2 tumor_only subworkflows
- [#1216](https://github.com/nf-core/sarek/pull/1216) - Better test coverage for variant calling `*_all` subworkflows

### Dependencies

Expand Down
Original file line number Diff line number Diff line change
@@ -1,5 +1,5 @@
//
// TUMOR VARIANT CALLING
// TUMOR ONLY VARIANT CALLING
// Should be only run on patients without normal sample
//

Expand Down
35 changes: 35 additions & 0 deletions tests/config/tags.yml
Original file line number Diff line number Diff line change
Expand Up @@ -207,6 +207,9 @@ cnvkit:
- modules/nf-core/cnvkit/batch/main.nf
- modules/nf-core/cnvkit/reference/main.nf
- subworkflows/local/bam_variant_calling_cnvkit/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- subworkflows/local/prepare_reference_cnvkit/main.nf
- tests/test_cnvkit.yml

Expand All @@ -222,7 +225,9 @@ controlfreec:
- modules/nf-core/controlfreec/makegraph/main.nf
- modules/nf-core/samtools/mpileup/main.nf
- subworkflows/local/bam_variant_calling_mpileup/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_controlfreec/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_controlfreec/main.nf
- tests/test_controlfreec.yml

Expand All @@ -233,6 +238,7 @@ deepvariant:
- modules/nf-core/gatk4/mergevcfs/main.nf
- modules/nf-core/tabix/tabix/main.nf
- subworkflows/local/bam_variant_calling_deepvariant/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- tests/test_deepvariant.yml

## freebayes
Expand All @@ -243,6 +249,9 @@ freebayes:
- modules/nf-core/gatk4/mergevcfs/main.nf
- modules/nf-core/tabix/tabix/main.nf
- subworkflows/local/bam_variant_calling_freebayes/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- tests/test_freebayes.yml

## haplotypecaller
Expand All @@ -255,6 +264,7 @@ haplotypecaller:
- modules/nf-core/samtools/index/main.nf
- modules/nf-core/samtools/merge/main.nf
- subworkflows/local/bam_merge_index_samtools/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
- subworkflows/local/vcf_variant_filtering_gatk/main.nf
- tests/test_haplotypecaller.yml
Expand All @@ -266,6 +276,7 @@ haplotypecaller_skip_filter:
- modules/nf-core/samtools/index/main.nf
- modules/nf-core/samtools/merge/main.nf
- subworkflows/local/bam_merge_index_samtools/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
- tests/test_haplotypecaller_skip_filter.yml

Expand All @@ -279,6 +290,7 @@ sentieon/haplotyper:
- modules/nf-core/samtools/index/main.nf
- modules/nf-core/samtools/merge/main.nf
- subworkflows/local/bam_merge_index_samtools/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
- subworkflows/local/vcf_variant_filtering_gatk/main.nf
- tests/test_sentieon_haplotyper.yml
Expand All @@ -290,6 +302,7 @@ sentieon_haplotyper_skip_filter:
- modules/nf-core/samtools/index/main.nf
- modules/nf-core/samtools/merge/main.nf
- subworkflows/local/bam_merge_index_samtools/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_sentieon_haplotyper/main.nf
- tests/test_sentieon_haplotyper_skip_filter.yml

Expand All @@ -309,6 +322,7 @@ joint_germline:
- modules/nf-core/samtools/merge/main.nf
- subworkflows/local/bam_joint_calling_germline_gatk/main.nf
- subworkflows/local/bam_merge_index_samtools/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
- subworkflows/local/vcf_variant_filtering_gatk/main.nf
- tests/test_joint_germline.yml
Expand All @@ -330,8 +344,11 @@ manta:
- modules/nf-core/manta/germline/main.nf
- modules/nf-core/manta/somatic/main.nf
- modules/nf-core/manta/tumoronly/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_germline_manta/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_manta/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_manta/main.nf
- tests/test_manta.yml

Expand All @@ -340,14 +357,18 @@ mpileup:
- conf/modules/mpileup.config
- modules/nf-core/cat/cat/main.nf
- modules/nf-core/samtools/mpileup/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_mpileup/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- tests/test_mpileup.yml

## msisensorpro
msisensorpro:
- conf/modules/msisensorpro.config
- modules/nf-core/msisensorpro/msi_somatic/main.nf
- modules/nf-core/msisensorpro/scan/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- tests/test_msisensorpro.yml

## mutect2
Expand All @@ -361,7 +382,9 @@ mutect2:
- modules/nf-core/gatk4/mergemutectstats/main.nf
- modules/nf-core/gatk4/mergevcfs/main.nf
- modules/nf-core/gatk4/mutect2/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_mutect2/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_mutect2/main.nf
- tests/test_mutect2.yml

Expand All @@ -371,8 +394,11 @@ strelka:
- modules/nf-core/gatk4/mergevcfs/main.nf
- modules/nf-core/strelka/germline/main.nf
- modules/nf-core/strelka/somatic/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_single_strelka/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- tests/test_strelka.yml

## strelka_bp
Expand All @@ -382,8 +408,11 @@ strelka_bp:
- modules/nf-core/gatk4/mergevcfs/main.nf
- modules/nf-core/manta/somatic/main.nf
- modules/nf-core/strelka/somatic/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_manta/main.nf
- subworkflows/local/bam_variant_calling_somatic_strelka/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- tests/test_strelka_bp.yml

## tiddit
Expand All @@ -392,8 +421,11 @@ tiddit:
- modules/nf-core/svdb/merge/main.nf.nf
- modules/nf-core/tabix/bgziptabix/main.nf
- modules/nf-core/tiddit/sv/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_single_tiddit/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_somatic_tiddit/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- tests/test_tiddit.yml

# annotate
Expand Down Expand Up @@ -453,11 +485,14 @@ concatenate_vcfs:
- modules/nf-core/tiddit/sv/main.nf
- subworkflows/local/bam_variant_calling_deepvariant/main.nf
- subworkflows/local/bam_variant_calling_freebayes/main.nf
- subworkflows/local/bam_variant_calling_germline_all/main.nf
- subworkflows/local/bam_variant_calling_germline_manta/main.nf
- subworkflows/local/bam_variant_calling_haplotypecaller/main.nf
- subworkflows/local/bam_variant_calling_mpileup/main.nf
- subworkflows/local/bam_variant_calling_single_strelka/main.nf
- subworkflows/local/bam_variant_calling_single_tiddit/main.nf
- subworkflows/local/bam_variant_calling_somatic_all/main.nf
- subworkflows/local/bam_variant_calling_tumor_only_all/main.nf
- subworkflows/local/post_variantcalling/main.nf
- subworkflows/local/vcf_concatenate_germline/main.nf
- tests/test_concat_germline_vcfs.yml