Skip to content

Commit

Permalink
NCBI Dataset field name transformations
Browse files Browse the repository at this point in the history
Originally the field map was created to keep mpox NDJSON backward compatible
with field names used from NCBI Virus. However, this constraint is not
applicable to dengue.

This commit organizes field renaming into two parts.

1. Rename the NCBI output columns to match the NCBI mnemonics
   (see `source-data/ncbi-dataset-field-map.tsv`)
2. Where necessary, rename the NCBI mnemonics to match Nextstrain expected column names
   (see "transform: fieldmap:" in `config/config.yaml`)

For context and discussion, see #13 (comment)
  • Loading branch information
j23414 committed Nov 8, 2023
1 parent 94b0113 commit c9caaab
Show file tree
Hide file tree
Showing 3 changed files with 38 additions and 26 deletions.
22 changes: 16 additions & 6 deletions ingest/config/config.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -10,14 +10,22 @@ transform:
# Fields to rename.
# This is the first step in the pipeline, so any references to field names
# in the configs below should use the new field names
field_map: ['collected=date', 'released=date_released', 'genbank_accession=accession', 'submitting_organization=institution']
field_map: [
'isolate-lineage=strain',
'sourcedb=database', # necessary for applying geo location rules
'geo-region=region',
'geo-location=location',
'isolate-collection-date=date',
'submitter-names=authors',
'submitter-affiliation=institution',
]
# Standardized strain name regex
# Currently accepts any characters because we do not have a clear standard for strain names
strain_regex: '^.+$'
# Back up strain name field if 'strain' doesn't match regex above
strain_backup_fields: ['accession']
# List of date fields to standardize
date_fields: ['date', 'date_released']
date_fields: ['date', 'release-date', 'update-date']
# Expected date formats present in date fields
# These date formats should use directives expected by datetime
# See https://docs.python.org/3.9/library/datetime.html#strftime-and-strptime-format-codes
Expand Down Expand Up @@ -55,16 +63,18 @@ transform:
# Final output columns for the metadata TSV
metadata_columns: [
'accession',
'genbank_accession_rev',
'accession-rev',
'strain',
'length',
'date',
'region',
'country',
'division',
'location',
'host',
'date_released',
'sra_accession',
'host-name',
'release-date',
'update-date',
'sra-accs',
'abbr_authors',
'authors',
'institution'
Expand Down
33 changes: 18 additions & 15 deletions ingest/source-data/ncbi-dataset-field-map.tsv
Original file line number Diff line number Diff line change
@@ -1,17 +1,20 @@
# Maps the NCBI output TSV column names back to the NCBI mnemonics.
# This list should match the list in
# ingest/workflow/snakemake_rules/fetch_sequences.smk _get_ncbi_dataset_field_mnemonics
key value
Accession genbank_accession_rev
Source database database
Isolate Lineage strain
Geographic Region region
Geographic Location location
Isolate Collection date collected
Release date released
Update date updated
Accession accession-rev
Source database sourcedb
Isolate Lineage isolate-lineage
Geographic Region geo-region
Geographic Location geo-location
Isolate Collection date isolate-collection-date
Release date release-date
Update date update-date
Length length
Host Name host
Isolate Lineage source isolation_source
BioProjects bioproject_accession
BioSample accession biosample_accession
SRA Accessions sra_accession
Submitter Names authors
Submitter Affiliation submitting_organization
Host Name host-name
Isolate Lineage source isolate-lineage-source
BioProjects bioprojects
BioSample accession biosample-acc
SRA Accessions sra-accs
Submitter Names submitter-names
Submitter Affiliation submitter-affiliation
9 changes: 4 additions & 5 deletions ingest/workflow/snakemake_rules/fetch_sequences.smk
Original file line number Diff line number Diff line change
Expand Up @@ -76,8 +76,7 @@ def _get_ncbi_dataset_field_mnemonics(wildcards) -> str:


rule format_ncbi_dataset_report:
# Formats the headers to be the same as before we used NCBI Datasets
# The only fields we do not have equivalents for are "title" and "publications"
# Formats the headers to match the NCBI mnemonic names
input:
dataset_package="data/ncbi_dataset.zip",
ncbi_field_map=config["ncbi_field_map"],
Expand All @@ -93,8 +92,8 @@ rule format_ncbi_dataset_report:
--package {input.dataset_package} \
--fields {params.fields_to_include:q} \
| csvtk -tl rename2 -F -f '*' -p '(.+)' -r '{{kv}}' -k {input.ncbi_field_map} \
| csvtk -tl mutate -f genbank_accession_rev -n genbank_accession -p "^(.+?)\." \
| tsv-select -H -f genbank_accession --rest last \
| csvtk -tl mutate -f accession-rev -n accession -p "^(.+?)\." \
| tsv-select -H -f accession --rest last \
> {output.ncbi_dataset_tsv}
"""

Expand All @@ -114,7 +113,7 @@ rule format_ncbi_datasets_ndjson:
augur curate passthru \
--metadata {input.ncbi_dataset_tsv} \
--fasta {input.ncbi_dataset_sequences} \
--seq-id-column genbank_accession_rev \
--seq-id-column accession-rev \
--seq-field sequence \
--unmatched-reporting warn \
--duplicate-reporting warn \
Expand Down

0 comments on commit c9caaab

Please sign in to comment.