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j23414 committed Feb 16, 2024
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Expand Up @@ -8,30 +8,43 @@ Follow the [standard installation instructions](https://docs.nextstrain.org/en/l

## Usage

> NOTE: All command examples assume you are within the `ingest` directory.
> If running commands from the outer `dengue` directory, please replace the `.` with `ingest`
All workflows are expected to the be run from the top level pathogen repo directory.
The default ingest workflow should be run with

Fetch sequences with

```sh
nextstrain build . data/sequences.ndjson
nextstrain build ingest ingest/data/sequences.ndjson
```

Run the complete ingest pipeline with

```sh
nextstrain build .
nextstrain build ingest
```

This will produce two files (within the `ingest` directory):
This will produce 10 files (within the `ingest` directory):

- `results/metadata.tsv`
- `results/sequences.fasta`
A pair of files with all the dengue sequences:

- `ingest/results/metadata_all.tsv`
- `ingest/results/sequences_all.fasta`

A pair of files for each dengue serotype (denv1 - denv4)

- `ingest/results/metadata_denv1.tsv`
- `ingest/results/sequences_denv1.fasta`
- `ingest/results/metadata_denv2.tsv`
- `ingest/results/sequences_denv2.fasta`
- `ingest/results/metadata_denv3.tsv`
- `ingest/results/sequences_denv3.fasta`
- `ingest/results/metadata_denv4.tsv`
- `ingest/results/sequences_denv4.fasta`

Run the complete ingest pipeline and upload results to AWS S3 with

```sh
nextstrain build . --configfiles config/config.yaml config/optional.yaml
nextstrain build ingest --configfiles config/config.yaml config/optional.yaml
```

### Adding new sequences not from GenBank
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