starCAT

Implements *CellAnnotator (aka *CAT/starCAT), annotating scRNA-Seq with predefined gene expression programs

Citation

If you use *CAT, please cite our preprint.

Installation

You can install starCAT and its dependencies via the Python Package Index.

pip install starcatpy

We tested it with scikit-learn 1.3.2, AnnData 0.9.2, and python 3.8. To run the tutorials, you also need jupyter or jupyterlab as well as scanpy and cnmf:

pip install jupyterlab scanpy cnmf

Basic starCAT usage

Please see our tutorials in python and R. A sample pipeline using a pre-built reference programs (TCAT.V1) is shown below.

# Load default TCAT reference from starCAT databse
tcat = starCAT(reference='TCAT.V1')

# tcat.ref.iloc[:5, :5]

#                     A1BG       AARD     AARSD1      ABCA1     ABCB1
# CellCycle-G2M   2.032614  22.965553  17.423538   3.478179  2.297279
# Translation    35.445282   0.000000   9.245893   0.477994  0.000000
# HLA            18.192997  14.632670   2.686475   3.937182  0.000000
# ISG             0.436212   0.000000  18.078197  17.354506  0.000000
# Mito           10.293049   0.000000  52.669895  14.615502  3.341488

# Load cell x genes counts data
adata = tcat.load_counts(datafn)

# Run starCAT
usage, scores = tcat.fit_transform(adata)

usage.iloc[0:2, 0:4]
#                             CellCycle-G2M  Translation       HLA       ISG
# CATGCCTAGTCGATAA-1-gPlexA4       0.000039     0.001042  0.001223  0.000162
# AAGACCTGTAGCGTCC-1-gPlexC6       0.000246     0.100023  0.002991  0.042354

scores.iloc[0:2, :]
#                                  ASA  Proliferation  ASA_binary  \
# CATGCCTAGTCGATAA-1-gPlexA4  0.001556        0.00052       False   
# AAGACCTGTAGCGTCC-1-gPlexC6  0.012503        0.01191       False   

#                             Proliferation_binary Multinomial_Label  
# CATGCCTAGTCGATAA-1-gPlexA4                 False         CD8_TEMRA  
# AAGACCTGTAGCGTCC-1-gPlexC6                 False         CD4_Naive  

starCAT also can be run in the command line.

starcat --reference "TCAT.V1" --counts {counts_fn} --output-dir {output_dir} --name {outuput_name}

• --reference - name of a default reference to download (ex. TCAT.V1) OR filepath containing a reference set of GEPs by genes (*.tsv/.csv/.txt), default is 'TCAT.V1'
• --counts - filepath to input (cell x gene) counts matrix as a matrix market (.mtx.gz), tab delimited text file, or anndata file (.h5ad)
• --scores - optional path to yaml file for calculating score add-ons, not necessary for pre-built references
• --output-dir - the output directory. all output will be placed in {output-dir}/{name}...'. default directory is '.'
• --name - the output analysis prefix name, default is 'starCAT'

Creating your own reference

We provide example scripts for constructing custom starCAT references from a single cNMF run or multiple cNMF runs.

Please let us know if you are interested in making your reference publically available for others to use analogous to our TCAT.V1 reference. You can email me at dkotliar@broadinstitute.org