diff --git a/packages/vite-node/src/utils.ts b/packages/vite-node/src/utils.ts
index b7673bb1c804..54931c2eed08 100644
--- a/packages/vite-node/src/utils.ts
+++ b/packages/vite-node/src/utils.ts
@@ -37,8 +37,7 @@ export function normalizeRequestId(id: string, base?: string): string {
     .replace(/^\/@id\/__x00__/, '\0') // virtual modules start with `\0`
     .replace(/^\/@id\//, '')
     .replace(/^__vite-browser-external:/, '')
-    .replace(/^file:/, '')
-    .replace(/^\/+/, '/') // remove duplicate leading slashes
+    .replace(/^file:(\/+)/, isWindows ? '' : '/') // remove file protocol and duplicate leading slashes
     .replace(/\?v=\w+/, '?') // remove ?v= query
     .replace(/&v=\w+/, '') // remove &v= query
     .replace(/\?t=\w+/, '?') // remove ?t= query
diff --git a/test/core/test/write-file-dynamic-import.test.ts b/test/core/test/write-file-dynamic-import.test.ts
new file mode 100644
index 000000000000..46cdaa2971a4
--- /dev/null
+++ b/test/core/test/write-file-dynamic-import.test.ts
@@ -0,0 +1,15 @@
+import { unlinkSync, writeFileSync } from 'node:fs'
+import { pathToFileURL } from 'node:url'
+import { afterEach, expect, it } from 'vitest'
+
+const filename = 'bar.js'
+
+afterEach(() => unlinkSync(filename))
+
+it('write file and import created file it should return created content.', async () => {
+  writeFileSync(filename, 'export default 123')
+
+  const mod = await import(pathToFileURL(filename).href)
+
+  expect(mod.default).toBe(123)
+})