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report.tsv empty #96

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deisyabril opened this issue Jun 27, 2016 · 2 comments
Closed

report.tsv empty #96

deisyabril opened this issue Jun 27, 2016 · 2 comments

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@deisyabril
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Hi, I installed all dependences for ARIBA and all the softwares are added to the path, all test passed and it installed correctly without errors.
Later, I downloaded the databases: CARD, argannot, ResFinder and VFDB, following the example in Wiki:
ariba getref card out.card
ariba prepareref --ref_prefix out.card out.card.prepareref
ariba run out.card.prepareref reads1.fastq reads2.fastq out.run

Nevertheless when I did the run task, I saw that the report.all.tsv file had many (-) symbols and the report.tsv file is empty.

result
report.all.tsv
report_all_tsv
report.tsv
report_tsv

How can I solve that?, thank you.

@martinghunt
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It's just noise, where a few reads have happened to map to some of the reference genes (see column D in report.all.tsv). None of them got assembled into anything matching the reference genes, hence the flags being 0 or 64 (column C). So when report.all.tsv is filtered to make report.tsv, there is nothing left to report.

@deisyabril
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Thanks for the information martinghunt,

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