Antigen affinity score

[sheridar]

No description provided.

[jayhesselberth]

Issues to consider:

Antigen-receptor affinity

• Should consider how monoclonal and polyclonal populations impact this score
• The best measures for calibration are Biacore analysis of recombinant antibody and antigen, also gives you k-on and k-off.

Receptor abundance

• Surface receptor levels could be measured explicit using CITE-seq with an antibody for heavy chain, which typically don't affect antigen binding (there's a name for these kinds of antibodies, Mia/JC use it).
• Could be predicted by BCR/TCR mRNA abundance

Staining protocol

• Ex vivo staining (e.g., LIBRA-seq and AVID-seq), done at 30 minutes at 4C.
• In vivo staining. I'm trying to convince somebody to do this, basically immunize mice with e.g. HEL-psDNA and score MD4 B cell acquisition levels.