diff --git a/lib/internal/crypto/webcrypto.js b/lib/internal/crypto/webcrypto.js
index cf440ebf8ff39d..3b7e4d13d8b1c0 100644
--- a/lib/internal/crypto/webcrypto.js
+++ b/lib/internal/crypto/webcrypto.js
@@ -6,6 +6,7 @@ const {
   JSONStringify,
   ObjectDefineProperties,
   ReflectApply,
+  ReflectConstruct,
   SafeSet,
   SymbolToStringTag,
   StringPrototypeRepeat,
@@ -31,6 +32,7 @@ const { TextDecoder, TextEncoder } = require('internal/encoding');
 
 const {
   codes: {
+    ERR_ILLEGAL_CONSTRUCTOR,
     ERR_INVALID_ARG_TYPE,
     ERR_INVALID_THIS,
   }
@@ -70,12 +72,21 @@ const {
   randomUUID: _randomUUID,
 } = require('internal/crypto/random');
 
-const randomUUID = () => _randomUUID();
+async function digest(algorithm, data) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
+  return ReflectApply(asyncDigest, this, arguments);
+}
+
+function randomUUID() {
+  if (this !== crypto) throw new ERR_INVALID_THIS('Crypto');
+  return _randomUUID();
+}
 
 async function generateKey(
   algorithm,
   extractable,
   keyUsages) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   algorithm = normalizeAlgorithm(algorithm);
   validateBoolean(extractable, 'extractable');
   validateArray(keyUsages, 'keyUsages');
@@ -123,6 +134,7 @@ async function generateKey(
 }
 
 async function deriveBits(algorithm, baseKey, length) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   algorithm = normalizeAlgorithm(algorithm);
   if (!isCryptoKey(baseKey))
     throw new ERR_INVALID_ARG_TYPE('baseKey', 'CryptoKey', baseKey);
@@ -194,6 +206,7 @@ async function deriveKey(
   derivedKeyAlgorithm,
   extractable,
   keyUsages) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   algorithm = normalizeAlgorithm(algorithm);
   derivedKeyAlgorithm = normalizeAlgorithm(derivedKeyAlgorithm);
   if (!isCryptoKey(baseKey))
@@ -238,7 +251,11 @@ async function deriveKey(
       throw lazyDOMException('Unrecognized name.');
   }
 
-  return importKey('raw', bits, derivedKeyAlgorithm, extractable, keyUsages);
+  return ReflectApply(
+    importKey,
+    this,
+    ['raw', bits, derivedKeyAlgorithm, extractable, keyUsages],
+  );
 }
 
 async function exportKeySpki(key) {
@@ -415,6 +432,7 @@ async function exportKeyJWK(key) {
 }
 
 async function exportKey(format, key) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   validateString(format, 'format');
   validateOneOf(format, 'format', kExportFormats);
   if (!isCryptoKey(key))
@@ -496,6 +514,7 @@ async function importKey(
   algorithm,
   extractable,
   keyUsages) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   validateString(format, 'format');
   validateOneOf(format, 'format', kExportFormats);
   if (format !== 'node.keyObject' && format !== 'jwk')
@@ -557,8 +576,9 @@ async function importKey(
 // subtle.wrapKey() is essentially a subtle.exportKey() followed
 // by a subtle.encrypt().
 async function wrapKey(format, key, wrappingKey, algorithm) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   algorithm = normalizeAlgorithm(algorithm);
-  let keyData = await exportKey(format, key);
+  let keyData = await ReflectApply(exportKey, this, [format, key]);
 
   if (format === 'jwk') {
     if (keyData == null || typeof keyData !== 'object')
@@ -586,6 +606,7 @@ async function unwrapKey(
   unwrappedKeyAlgo,
   extractable,
   keyUsages) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   wrappedKey = getArrayBufferOrView(wrappedKey, 'wrappedKey');
   unwrapAlgo = normalizeAlgorithm(unwrapAlgo);
   let keyData = await cipherOrWrap(
@@ -607,7 +628,11 @@ async function unwrapKey(
     }
   }
 
-  return importKey(format, keyData, unwrappedKeyAlgo, extractable, keyUsages);
+  return ReflectApply(
+    importKey,
+    this,
+    [format, keyData, unwrappedKeyAlgo, extractable, keyUsages],
+  );
 }
 
 function signVerify(algorithm, key, data, signature) {
@@ -654,10 +679,12 @@ function signVerify(algorithm, key, data, signature) {
 }
 
 async function sign(algorithm, key, data) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   return signVerify(algorithm, key, data);
 }
 
 async function verify(algorithm, key, signature, data) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   return signVerify(algorithm, key, data, signature);
 }
 
@@ -707,30 +734,39 @@ async function cipherOrWrap(mode, algorithm, key, data, op) {
 }
 
 async function encrypt(algorithm, key, data) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   return cipherOrWrap(kWebCryptoCipherEncrypt, algorithm, key, data, 'encrypt');
 }
 
 async function decrypt(algorithm, key, data) {
+  if (this !== subtle) throw new ERR_INVALID_THIS('SubtleCrypto');
   return cipherOrWrap(kWebCryptoCipherDecrypt, algorithm, key, data, 'decrypt');
 }
 
 // The SubtleCrypto and Crypto classes are defined as part of the
 // Web Crypto API standard: https://www.w3.org/TR/WebCryptoAPI/
 
-class SubtleCrypto {}
-const subtle = new SubtleCrypto();
+class SubtleCrypto {
+  constructor() {
+    throw new ERR_ILLEGAL_CONSTRUCTOR();
+  }
+}
+const subtle = ReflectConstruct(function() {}, [], SubtleCrypto);
 
 class Crypto {
+  constructor() {
+    throw new ERR_ILLEGAL_CONSTRUCTOR();
+  }
+
   get subtle() {
+    if (this !== crypto) throw new ERR_INVALID_THIS('Crypto');
     return subtle;
   }
 }
-const crypto = new Crypto();
+const crypto = ReflectConstruct(function() {}, [], Crypto);
 
 function getRandomValues(array) {
-  if (!(this instanceof Crypto)) {
-    throw new ERR_INVALID_THIS('Crypto');
-  }
+  if (this !== crypto) throw new ERR_INVALID_THIS('Crypto');
   return ReflectApply(_getRandomValues, this, arguments);
 }
 
@@ -799,7 +835,7 @@ ObjectDefineProperties(
       enumerable: true,
       configurable: true,
       writable: true,
-      value: asyncDigest,
+      value: digest,
     },
     generateKey: {
       enumerable: true,
diff --git a/test/parallel/test-webcrypto-constructors.js b/test/parallel/test-webcrypto-constructors.js
new file mode 100644
index 00000000000000..ddc4e8cb0796b1
--- /dev/null
+++ b/test/parallel/test-webcrypto-constructors.js
@@ -0,0 +1,159 @@
+// Flags: --experimental-global-webcrypto
+'use strict';
+
+const common = require('../common');
+
+if (!common.hasCrypto)
+  common.skip('missing crypto');
+
+const assert = require('assert');
+
+// Test CryptoKey constructor
+{
+  assert.throws(() => new CryptoKey(), {
+    name: 'TypeError', message: 'Illegal constructor', code: 'ERR_ILLEGAL_CONSTRUCTOR'
+  });
+}
+
+// Test SubtleCrypto constructor
+{
+  assert.throws(() => new SubtleCrypto(), {
+    name: 'TypeError', message: 'Illegal constructor', code: 'ERR_ILLEGAL_CONSTRUCTOR'
+  });
+}
+
+// Test Crypto constructor
+{
+  assert.throws(() => new Crypto(), {
+    name: 'TypeError', message: 'Illegal constructor', code: 'ERR_ILLEGAL_CONSTRUCTOR'
+  });
+}
+
+const notCrypto = Reflect.construct(function() {}, [], Crypto);
+const notSubtle = Reflect.construct(function() {}, [], SubtleCrypto);
+
+// Test Crypto.prototype.subtle
+{
+  assert.throws(() => notCrypto.subtle, {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  });
+}
+
+// Test Crypto.prototype.randomUUID
+{
+  assert.throws(() => notCrypto.randomUUID(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  });
+}
+
+// Test Crypto.prototype.getRandomValues
+{
+  assert.throws(() => notCrypto.getRandomValues(new Uint8Array(12)), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  });
+}
+
+// Test SubtleCrypto.prototype.encrypt
+{
+  assert.rejects(() => notSubtle.encrypt(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.decrypt
+{
+  assert.rejects(() => notSubtle.decrypt(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.sign
+{
+  assert.rejects(() => notSubtle.sign(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.verify
+{
+  assert.rejects(() => notSubtle.verify(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.digest
+{
+  assert.rejects(() => notSubtle.digest(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.generateKey
+{
+  assert.rejects(() => notSubtle.generateKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.deriveKey
+{
+  assert.rejects(() => notSubtle.deriveKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.deriveBits
+{
+  assert.rejects(() => notSubtle.deriveBits(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.importKey
+{
+  assert.rejects(() => notSubtle.importKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.exportKey
+{
+  assert.rejects(() => notSubtle.exportKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.wrapKey
+{
+  assert.rejects(() => notSubtle.wrapKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+// Test SubtleCrypto.prototype.unwrapKey
+{
+  assert.rejects(() => notSubtle.unwrapKey(), {
+    name: 'TypeError', code: 'ERR_INVALID_THIS',
+  }).then(common.mustCall());
+}
+
+{
+  globalThis.crypto.subtle.importKey(
+    'raw',
+    globalThis.crypto.getRandomValues(new Uint8Array(4)),
+    'PBKDF2',
+    false,
+    ['deriveKey'],
+  ).then((key) => {
+    globalThis.crypto.subtle.importKey = common.mustNotCall();
+    return globalThis.crypto.subtle.deriveKey({
+      name: 'PBKDF2',
+      hash: 'SHA-512',
+      salt: globalThis.crypto.getRandomValues(new Uint8Array()),
+      iterations: 5,
+    }, key, {
+      name: 'AES-GCM',
+      length: 256
+    }, true, ['encrypt', 'decrypt']);
+  }).then(common.mustCall());
+}