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Co-authored-by: Jonathan Manning <pininforthefjords@gmail.com>
Co-authored-by: Adam Talbot <12817534+adamrtalbot@users.noreply.github.com>
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3 people committed Mar 20, 2024
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Expand Up @@ -78,11 +78,11 @@ To use this approach, include the transcript lengths file with the **raw counts*
--transcript_length_matrix 'salmon.merged.gene_lengths.tsv'
```

Without the transcript lengths, for instance in earlier RNAseq workflow versions, follow the second recommendation in the [tximport documentation](https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html#Downstream_DGE_in_Bioconductor):
Without the transcript lengths, for instance in earlier nf-core/rnaseq workflow versions, follow the second recommendation in the [tximport documentation](https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html#Downstream_DGE_in_Bioconductor):

> "Use the tximport argument `countsFromAbundance='lengthScaledTPM'` or `'scaledTPM'`, then employ the gene-level count matrix `txi$counts` directly in downstream software, a method we call 'bias corrected counts without an offset'"
This aligns with the **gene_counts_length_scaled.tsv** or **gene_counts_scaled.tsv** matrices in the RNAseq workflow.
This aligns with the **gene_counts_length_scaled.tsv** or **gene_counts_scaled.tsv** matrices in the nf-core/rnaseq workflow.

It is important to note that the documentation advises:

Expand Down Expand Up @@ -158,7 +158,7 @@ The file can be tab or comma separated.
--gtf '[path to gtf file]'
```

This is usually the easiest way to supply annotations for RNA-seq features. It should match the GTF used in nf-core/RNAseq if that workflow was used to produce the input expression matrix. Skip for MaxQuant.
This is usually the easiest way to supply annotations for RNA-seq features. It should match the GTF used in nf-core/rnaseq if that workflow was used to produce the input expression matrix. Skip for MaxQuant.

### Annotation package identifiers for Affymetrix arrays

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