diff --git a/boards/st/b_u585i_iot02a/b_u585i_iot02a-common.dtsi b/boards/st/b_u585i_iot02a/b_u585i_iot02a-common.dtsi
index d6f4ebfe7e7db..9c0dc5e26616b 100644
--- a/boards/st/b_u585i_iot02a/b_u585i_iot02a-common.dtsi
+++ b/boards/st/b_u585i_iot02a/b_u585i_iot02a-common.dtsi
@@ -152,8 +152,19 @@ stm32_lp_tick_source: &lptim1 {
 			#address-cells = <1>;
 			#size-cells = <1>;
 
-			partition@0 {
-				reg = <0x00000000 DT_SIZE_M(64)>;
+			slot0_partition: partition@0 {
+				label = "image-0";
+				reg = <0x00000000 DT_SIZE_K(256)>;
+			};
+
+			slot1_partition: partition@40000 {
+				label = "image-1";
+				reg = <0x00040000 DT_SIZE_K(256)>;
+			};
+
+			scratch_partition: partition@e0000 {
+				label = "image-scratch";
+				reg = <0x000e0000 DT_SIZE_K(64)>;
 			};
 		};
 	};
diff --git a/boards/st/b_u585i_iot02a/b_u585i_iot02a.dts b/boards/st/b_u585i_iot02a/b_u585i_iot02a.dts
index fc824417d2050..6347cfad85f8f 100644
--- a/boards/st/b_u585i_iot02a/b_u585i_iot02a.dts
+++ b/boards/st/b_u585i_iot02a/b_u585i_iot02a.dts
@@ -54,21 +54,6 @@
 			reg = <0x00000000 DT_SIZE_K(64)>;
 		};
 
-		slot0_partition: partition@10000 {
-			label = "image-0";
-			reg = <0x00010000 DT_SIZE_K(416)>;
-		};
-
-		slot1_partition: partition@78000 {
-			label = "image-1";
-			reg = <0x00078000 DT_SIZE_K(416)>;
-		};
-
-		scratch_partition: partition@e0000 {
-			label = "image-scratch";
-			reg = <0x000e0000 DT_SIZE_K(64)>;
-		};
-
 		storage_partition: partition@f0000 {
 			label = "storage";
 			reg = <0x000f0000 DT_SIZE_K(64)>;
diff --git a/boards/st/b_u585i_iot02a/b_u585i_iot02a.yaml b/boards/st/b_u585i_iot02a/b_u585i_iot02a.yaml
index 621e455720497..93ff03bda277f 100644
--- a/boards/st/b_u585i_iot02a/b_u585i_iot02a.yaml
+++ b/boards/st/b_u585i_iot02a/b_u585i_iot02a.yaml
@@ -12,7 +12,6 @@ supported:
   - arduino_spi
   - hts221
   - dma
-  - usb_device
   - spi
   - dac
   - adc
diff --git a/boards/st/b_u585i_iot02a/board.cmake b/boards/st/b_u585i_iot02a/board.cmake
index bb801eaae547d..5764f61791382 100644
--- a/boards/st/b_u585i_iot02a/board.cmake
+++ b/boards/st/b_u585i_iot02a/board.cmake
@@ -13,11 +13,9 @@ if(CONFIG_BUILD_WITH_TFM)
 endif()
 
 # keep first
-if(CONFIG_STM32_MEMMAP)
 board_runner_args(stm32cubeprogrammer "--port=swd" "--reset-mode=hw")
-board_runner_args(stm32cubeprogrammer "--extload=MX25LM51245G_STM32U585I-IOT02A.stldr")
-else()
-board_runner_args(stm32cubeprogrammer "--erase" "--port=swd" "--reset-mode=hw")
+if(CONFIG_STM32_MEMMAP OR (CONFIG_XIP AND CONFIG_BOOTLOADER_MCUBOOT))
+  board_runner_args(stm32cubeprogrammer "--extload=MX25LM51245G_STM32U585I-IOT02A.stldr")
 endif()
 
 board_runner_args(openocd "--tcl-port=6666")
diff --git a/samples/sysbuild/with_mcuboot/boards/b_u585i_iot02a.overlay b/samples/sysbuild/with_mcuboot/boards/b_u585i_iot02a.overlay
new file mode 100644
index 0000000000000..f109c447c637e
--- /dev/null
+++ b/samples/sysbuild/with_mcuboot/boards/b_u585i_iot02a.overlay
@@ -0,0 +1,17 @@
+/*
+ * Copyright (c) 2025 STMicroelectronics
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/*
+ * Define the device, controller and partition to be the external memory
+ * for running the application in external NOR from MCUboot
+ */
+
+/ {
+	chosen {
+		zephyr,flash = &mx25lm51245;
+		zephyr,flash-controller = &mx25lm51245;
+	};
+};
diff --git a/samples/sysbuild/with_mcuboot/sample.yaml b/samples/sysbuild/with_mcuboot/sample.yaml
index a2e53a4fbecb0..ec72ef4e2f8bd 100644
--- a/samples/sysbuild/with_mcuboot/sample.yaml
+++ b/samples/sysbuild/with_mcuboot/sample.yaml
@@ -17,6 +17,7 @@ tests:
       - nucleo_h7s3l8
       - nucleo_u385rg_q
       - stm32h7s78_dk
+      - b_u585i_iot02a
     integration_platforms:
       - nrf52840dk/nrf52840
       - esp32_devkitc/esp32/procpu
diff --git a/soc/st/stm32/Kconfig.defconfig b/soc/st/stm32/Kconfig.defconfig
index aafb321151a46..e18bc5d5bbadb 100644
--- a/soc/st/stm32/Kconfig.defconfig
+++ b/soc/st/stm32/Kconfig.defconfig
@@ -28,10 +28,12 @@ DT_STM32_LPTIM_PATH := $(dt_nodelabel_path,stm32_lp_tick_source)
 
 DT_CHOSEN_Z_FLASH := zephyr,flash
 DT_COMPAT_XSPI := st,stm32-xspi
+DT_COMPAT_OSPI := st,stm32-ospi
 
 DT_CHOSEN_FLASH_NODE := $(dt_chosen_path,$(DT_CHOSEN_Z_FLASH))
 DT_CHOSEN_FLASH_PARENT := $(dt_node_parent,$(DT_CHOSEN_FLASH_NODE))
 DT_FLASH_PARENT_IS_XSPI := $(dt_node_has_compat,$(DT_CHOSEN_FLASH_PARENT),$(DT_COMPAT_XSPI))
+DT_FLASH_PARENT_IS_OSPI := $(dt_node_has_compat,$(DT_CHOSEN_FLASH_PARENT),$(DT_COMPAT_OSPI))
 
 config SYS_CLOCK_HW_CYCLES_PER_SEC
 	default "$(DT_STM32_RCC_CLOCK_FREQ)" if "$(dt_nodelabel_enabled,rcc)"
@@ -82,7 +84,7 @@ config BUILD_WITH_TFM
 
 config FLASH_BASE_ADDRESS
 	default $(dt_node_reg_addr_hex,$(DT_CHOSEN_FLASH_PARENT),1) \
-		if $(DT_FLASH_PARENT_IS_XSPI)
+		if $(DT_FLASH_PARENT_IS_XSPI) || $(DT_FLASH_PARENT_IS_OSPI)
 	default $(dt_chosen_reg_addr_hex,$(DT_CHOSEN_Z_FLASH))
 
 # The XSPI PSRAM driver creates a SMH region with attribute SMH_REG_ATTR_EXTERNAL (2)